Advances in HPLC-ICP-MS interface techniques for metal speciation
- Univ. of Plymouth (United Kingdom)
The relentless demand for lower detection limits is increasingly coupled to the requirement for elemental speciation. This is particularly true in environmental and clinical fields where total levels are often insufficient for mobility and toxicity studies. This demand for both qualitative and quantitative data on the individual species present in complex samples has led to the development of various interfaces to couple some form of chromatography, usually gas chromatography (GC) or high performance liquid chromatography (HPLC) to an element specific detector. Today inductively coupled plasma-mass spectrometry is often employed since it offers excellent detection limits, element specific information (including isotopic data) and the potential for multi-element studies. Ms presentation will concentrate on HPLC couplings although the advantages and disadvantages of both GC and HPLC couplings to ICP-MS will be discussed. Particular attention will be given to the optimization of both the chromatography and detection systems. Details will be presented of several successful HPLC interface designs and ways of facilitating high levels of a range of organic solvents (e.g. methanol and THF) in the HPLC mobile phase will be highlighted. The advantages of using a sheath gas and practical ways of achieving this will also be discussed. Finally the use of isotope dilution analysis in conjunction with HPLC-ICP-MS will be outlined. In all cases the impact of using the most appropriate approach will be demonstrated using both environmental and clinical samples.
- OSTI ID:
- 466509
- Report Number(s):
- CONF-951017-; TRN: 96:005999-0041
- Resource Relation:
- Conference: 22. annual conference of the Federation of Analytical Chemistry and Spectroscopy Societies, Cincinnati, OH (United States), 15-20 Oct 1995; Other Information: PBD: 1995; Related Information: Is Part Of FACSS XXII - the 22nd annual conference of the Federation of Analytical Chemistry and Spectroscopy Societies; PB: 285 p.
- Country of Publication:
- United States
- Language:
- English
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