A 350-kb cosmid contig in 3p14.2 that crosses the t(3;8) hereditary renal cell carcinoma translocation breakpoint and 17 aphidicolin-induced FRA3B breakpoints
- Wayne State Univ. School of Medicine, Detroit, MI (United States)
- Univ. of Michigan Medical School, Ann Arbor, MI (United States); and others
The constitutive fragile site at human chromosomal band 3p14.2, FRA3B, has been described as the most active common fragile site in the human genome. Previous work demonstrated that a 1330-kb YAC clone, YC850A6, spans both the t(3;8) translocation and FRA3B and also encompasses FRA3B-associated breakpoints was used to construct a multi-hit cosmid library. Screening of this library resulted in a 350-kb cosmid contig that extends distally from the t(3;8) translocation breakpoint. Seventeen aphidicolin-induced 3p14.2 breakpoints derived from hamster-human hybrids were mapped within this cosmid contig. These breakpoints were found to localize as two distinct clusters, separated by 200 kb, which lie on either side of a region of frequent breakage within FRA3B as defined by FISH analysis using cosmids from the contigs. The distribution of these breakpoints, together with the region of frequent chromosomal breakage mapped by FISH analysis, further confirms the position of FRA3B comprises several hundred kilobases of DNA sequence within 3p14.2. The 350-kb contig and the cosmid library constructed from YAC YC850A6 will be essential for further characterization of the region surrounding FRA3B and in experiments to determine the molecular basis of the fragility of FRA3B.
- OSTI ID:
- 466022
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 1 Vol. 35; ISSN 0888-7543; ISSN GNMCEP
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BASIC STUDIES
CARCINOMAS
CHROMOSOMAL ABERRATIONS
CHROMOSOME BREAKAGE
CONTIGS
COSMIDS
DNA REPLICATION
DNA SEQUENCING
DNA-CLONING
FLUORESCENCE
GENETIC MAPPING
HEREDITARY DISEASES
HUMAN CHROMOSOME 3
HUMAN CHROMOSOME 8
HYBRIDIZATION
IN-SITU HYBRIDIZATION
MUTATION FREQUENCY
PATIENTS
POLYMERASE CHAIN REACTION
SOMATIC CELLS
UROGENITAL SYSTEM DISEASES