RADIATION EFFECT ON THE RNA METABOLISM OF THE CENTRAL NERVOUS SYSTEM

Technical Report · Fri Dec 21 04:00:00 EST 1962

OSTI ID:4659949

Yamamoto, Y L; Feinendegen, L E; Bond, V P

Tritiated cytidine was used to study autoradiographically the effect of 500- and 15,000-r x ray (250-kvp) irradiation on RNA synthesis and metabolism in the mature fully developed central nervous system of the rat. The label was injected intravenously immediately following irradiation. Local irradiation of the head and 500-r irradiation of the total body resulted in a depression of cytoplasmic RNA labeling, 24 to 48 hr after exposure in the choroid plexus cells, pyramidal cells (Rose's area h/sup 3/) in the hippocampus, neurons in the third layer of the postcentral cortex, and Purkinje cells of the cerebellum. Labeling of nuclear RNA immediately after irradiation was, however, not sufficiently different in experimental and control animals to account for the subsequent difference in the cytoplasmic labeling. The decline with time of nuclear RNA labeling was rather parallel in all groups. Five hundred r irradiation to the total body resulted in a greater depression of cytoplasmic RNA labeling in the brain cells than did 500 r localized to the head. The described responses were of similar magnitude in the different neurons examined. There were no morphological changes in the cell systems described following irradiation with 500 r. (auth)

• Research Organization: Brookhaven National Lab., Upton, N.Y.
• DOE Contract Number: AT(30-2)-GEN-16
• NSA Number: NSA-17-037060
• OSTI ID: 4659949
• Report Number(s): BNL-6600
• Country of Publication: United States
• Language: English

Related Subjects

AGE
ANIMAL CELLS
BIOCHEMISTRY
BIOLOGY AND MEDICINE
BRAIN
CYTIDINE
LABELLED COMPOUNDS
METABOLISM
NEUTRONS
RADIATION DOSES
RADIATION EFFECTS
RADIATION INJURIES
RATS
RIBONUCLEIC ACID
TRACER TECHNIQUES
TRITIUM
X RADIATION