Cloning of cDNAs encoding the human BAG1 protein and localization of the human BAG1 gene to chromosome 9p12

Journal Article · Thu Aug 01 04:00:00 EDT 1996 · Genomics

DOI:https://doi.org/10.1006/geno.1996.0389· OSTI ID:465984

Takayama, Shinichi; Kochel, K; Irie, Shinji ^[1]

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1. Burnham Institute, La Jolla, CA (United States); and others

cDNAs encoding the human homolog of BAG1, a Bcl-2-binding protein with anti-apoptotic function, were cloned. DNA sequence analysis of human BAG1 cDNAs predicts a protein with an additional 55 amino acids at its NH2-terminus compared to the mouse protein. Immunoblot assays using monoclonal antibodies raised against bacterially produced h-BAG1 protein confirmed the larger size of the human protein ({approximately}34 kDa) compared to mouse. PCR analysis of DNA form human x rodent somatic cell hybrids using human BAG1-specific primers localized the gene to human chromosome 9. Cosmid clones of h-BAG1 were obtained and used for fluorescence in situ hybridization analysis of normal metaphase chromosomes, thus localizing h-BAG1 to 9p12, a region associated with hereditary disorders that may involve developmental dysregulation of programmed cell death. 15 refs., 4 figs.

• OSTI ID: 465984
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 3 Vol. 35; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
AMINO ACID SEQUENCE
ANIMAL CELLS
CELL KILLING
CHROMOSOMAL ABERRATIONS
COSMIDS
DNA SEQUENCING
DNA-CLONING
FLUORESCENCE
GENE REGULATION
GENES
GENETIC MAPPING
HEREDITARY DISEASES
HUMAN CHROMOSOME 9
HUMAN CHROMOSOMES
HYBRIDIZATION
IN-SITU HYBRIDIZATION
LYMPHOMAS
MAN
POLYMERASE CHAIN REACTION
PROTEINS
SOMATIC CELLS