Sequence, organization, and transcription of the Dlx-1 and Dlx-2 locus

Journal Article · Thu Aug 01 00:00:00 EDT 1996 · Genomics

DOI:https://doi.org/10.1006/geno.1996.0387· OSTI ID:465982

McGuinness, T; Porteus, M H; Smiga, S; Bulfone, A ^[1]

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1. Univ. of California, San Francisco, CA (United States); and others

There are at least five murine Dlx genes that are related to the Drosophila Distal-less homeobox gene. The Dlx genes are primarily expressed in the developing forebrain, derivatives of the cranial neutral crest and restricted epidermal craniofacial and limb domains. Dlx-2 is required to differentiation of subsets of cranial neural crest and forebrain cells. Previous genomic studies have shown that Dlx-1 and Dlx-2 are linked on mouse chromosome 2, near the HoxD cluster. Here we report a detailed analysis of the nucleotide sequence ({approximately} 14 kb), organization, and transcription of the murine Dlx-1 and Dlx-2 locus. In addition, we show that Dlx-1 makes multiple sense transcripts and at least one antisense transcript, whereas Dlx-2 makes one major transcript. The sequence of the human Dlx-2 gene is reported and is compared to that of the murine gene. Finally, sequence analysis of the deduced protein sequences reveals several candidate functional domains. 58 refs., 9 figs., 1 tab.

• OSTI ID: 465982
• Journal Information: Genomics, Vol. 35, Issue 3; Other Information: PBD: 1 Aug 1996
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
GENES
DNA SEQUENCING
STRUCTURE-ACTIVITY RELATIONSHIPS
TRANSCRIPTION
GENE REGULATION
GENETIC MAPPING
GENE MUTATIONS
DNA-CLONING
BRAIN
ONTOGENESIS
ANIMAL CELLS
CELL DIFFERENTIATION
CHROMOSOMES
CORRELATIONS
HUMAN CHROMOSOME 2
MICE
DROSOPHILA
PROTEINS
NUCLEOTIDES
POLYMERASE CHAIN REACTION
PROBES
AMINO ACID SEQUENCE