THE DISTRIBUTION OF Rb$sup 86$ AND Cs$sup 134$ IN SOME TISSUES OF THE DOG AFTER INTRAVENOUS INJECTION, WITH SPECIAL REFERENCE TO RENAL UPTAKE
Decay studies on Rb/sup 86/ revealed the presence of an impurity with a long half-life. Pulse height analysis established that this was due to contamination with Cs/sup 134/. It was subsequently confirmed by the Hadiochemical Centre (Amersham) that up to 2% contamination with Cs/sup 134/ might be present at the time of delivery of the isotope, under which circumstances, at 2 weeks after delivery 40% of the emission detected would be coming from the contaminant. Since Rb/sup 86/ was being used with external counting for differential renal blood flow studies in man, it seemed possible that this might affect the accuracy of the results. Consequently studies were carried out to investigate tissue distribution of these isotopes after intravenous injection in the dog. Rb/sup 86/ or Cs/sup 134/ was given intravenously to dogs, and at intervals of 1-30 min the animals were killed, immediately after clamping the great vessels in the lower chest. Activity per g of kidney, liver, spleen, muscle, and blood was determined as a fraction of the dose injected. One kidney was exposed, and with a heavily shielded scintillation counter, counts were taken at 15-sec intervals after injection of Rb/sup 86/ or Cs/sup 134/. Curves were obtained to provide a comparison of the variation with time of the concentration of these isotopes in kidney. The concentrations of Rb and Cs in the liver, spleen, and muscle were similar during the 30 min following intravenous injection. Max renal uptake of Rb occurred at 1-2 min and of Cs at 10-20 min, when their respective concentrations in the kidney were of the order of 10 times those in the other tissues measured. Blood concentrations of Rb had fallen to tissue levels at 1-2 min, while blood Cs concentration at this time was still as high as that in the kidney. These studies show that Cs contamination of Rb in the degree noted would significantly reduce the accuracy of the method. Cs- free Rb was later made available, and newer findings suggest that differential renal blood flow studies using this purer isotope are valid. (BBB)
- Research Organization:
- London Hospital
- NSA Number:
- NSA-17-035428
- OSTI ID:
- 4659716
- Journal Information:
- Journal of Physiology (London) (England), Vol. Vol: 166; Other Information: Orig. Receipt Date: 31-DEC-63
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
Similar Records
LOCALIZATION OF RADIOACTIVITY BY INTRAVASCULAR INJECTION OF LARGE RADIOACTIVE PARTICLES
SEMIANNUAL PROGRESS REPORT FOR THE PERIOD ENDING DECEMBER 31, 1960