A high-resolution whole genome radiation hybrid map of human chromosome 17q22-q25.3 across the genes for GH and TK

Journal Article · Mon Apr 15 00:00:00 EDT 1996 · Genomics

DOI:https://doi.org/10.1006/geno.1996.0182· OSTI ID:465947

Foster, J W; Schafer, A J; Critcher, R ^[1]

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1. Univ. of Cambridge (United Kingdom); and others

We have constructed a whole genome radiation hybrid (WG-RH) map across a region of human chromosome 17q, from growth hormone (GH) to thymidine kinase (TK). A panel of 128 WG-RH hybrid cell lines generated by X-irradiation and fusion has been tested for the retention of 39 sequence-tagged site (STS) markers by the polymerase chain reaction. This genome mapping technique has allowed the integration of existing VNTR and microsatellite markers with additional new markers and existing STS markers previously mapped to this region by other means. The WG-RH map includes eight expressed sequence tag (EST) and three anonymous markers developed for this study, together with 23 anonymous microsatellites and five existing ESTs. Analysis of these data resulted in a high-density comprehensive map across this region of the genome. A subset of these markers has been used to produce a framework map consisting of 20 loci ordered with odds greater than 1000:1. The markers are of sufficient density to build a YAC contig across this region based on marker content. We have developed sequence tags for both ends of a 2.1-Mb YAC and mapped these using the WG-RH panel, allowing a direct comparison of cRay{sub 6000} to physical distance. 31 refs., 3 figs., 2 tabs.

• OSTI ID: 465947
• Journal Information: Genomics, Vol. 33, Issue 2; Other Information: PBD: 15 Apr 1996
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
99 MATHEMATICS
COMPUTERS
INFORMATION SCIENCE
MANAGEMENT
LAW
MISCELLANEOUS
HUMAN CHROMOSOME 17
GENETIC MAPPING
DNA SEQUENCING
RESOLUTION
COORDINATED RESEARCH PROGRAMS
MAXIMUM-LIKELIHOOD FIT
GENES
CONTIGS
DESIGN
MAN
HEREDITARY DISEASES
RADIATION INDUCED MUTANTS
HYBRIDIZATION
BIOLOGICAL MARKERS
STH
THYMIDINE
PHOSPHOTRANSFERASES
POLYMERASE CHAIN REACTION
STATISTICS