A high-resolution whole genome radiation hybrid map of human chromosome 17q22-q25.3 across the genes for GH and TK
- Univ. of Cambridge (United Kingdom); and others
We have constructed a whole genome radiation hybrid (WG-RH) map across a region of human chromosome 17q, from growth hormone (GH) to thymidine kinase (TK). A panel of 128 WG-RH hybrid cell lines generated by X-irradiation and fusion has been tested for the retention of 39 sequence-tagged site (STS) markers by the polymerase chain reaction. This genome mapping technique has allowed the integration of existing VNTR and microsatellite markers with additional new markers and existing STS markers previously mapped to this region by other means. The WG-RH map includes eight expressed sequence tag (EST) and three anonymous markers developed for this study, together with 23 anonymous microsatellites and five existing ESTs. Analysis of these data resulted in a high-density comprehensive map across this region of the genome. A subset of these markers has been used to produce a framework map consisting of 20 loci ordered with odds greater than 1000:1. The markers are of sufficient density to build a YAC contig across this region based on marker content. We have developed sequence tags for both ends of a 2.1-Mb YAC and mapped these using the WG-RH panel, allowing a direct comparison of cRay{sub 6000} to physical distance. 31 refs., 3 figs., 2 tabs.
- OSTI ID:
- 465947
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 33; ISSN 0888-7543; ISSN GNMCEP
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BASIC STUDIES
99 GENERAL AND MISCELLANEOUS
BIOLOGICAL MARKERS
CONTIGS
COORDINATED RESEARCH PROGRAMS
DESIGN
DNA SEQUENCING
GENES
GENETIC MAPPING
HEREDITARY DISEASES
HUMAN CHROMOSOME 17
HYBRIDIZATION
MAN
MAXIMUM-LIKELIHOOD FIT
PHOSPHOTRANSFERASES
POLYMERASE CHAIN REACTION
RADIATION INDUCED MUTANTS
RESOLUTION
STATISTICS
STH
THYMIDINE