Framework for a physical map of the human 22q13 region using bacterial artificial chromosomes (BACs)

Journal Article · Mon Apr 01 04:00:00 EST 1996 · Genomics

DOI:https://doi.org/10.1006/geno.1996.0154· OSTI ID:465920

Schmitt, H; Kim, Ung-Jin; Slepak, T ^[1]

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1. California Institute of Technology, Pasadena, CA (United States); and others

Detailed physical maps of entire chromosomes based on combined genetic, cytogenetic, and structural information are essential components for positional cloning and genomic sequencing. Despite the wealth of genetic information of the known diseases in the chromosome 22q13 region, the construction of a detailed physical map of the terminal region is difficult due to the sparsity of the genetic markers. We present here a map of bacterial artificial chromosome (BAC) contigs that cover a number of genetic loci in the 22q13 region. One hundred thirty-six BACs with an average insert size of 140 kb are assembled into 35 contigs defined by 64 markers in 22q13-qter. Twenty-three anonymous markers are now linked to the previously mapped genetic anchor points. 55 refs., 4 figs., 3 tabs.

• DOE Contract Number: FG03-89ER60891
• OSTI ID: 465920
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 1 Vol. 33; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
BIOLOGICAL MARKERS
CHROMOSOMAL ABERRATIONS
CONTIGS
DESIGN
DNA SEQUENCING
DNA-CLONING
ESCHERICHIA COLI
GENES
GENETIC MAPPING
HEREDITARY DISEASES
HUMAN CHROMOSOME 22
LEUKEMIA
MAN
NEOPLASMS
PLASMIDS
POLYMERASE CHAIN REACTION
PROBES
STRUCTURE-ACTIVITY RELATIONSHIPS