The effects of ultraviolet-irradiated polyuridylic acid in cell-free protein synthesis in escherichia coli.II. The influence of specific photoproducts
Two uracil photoproducts are formed when polyuridylic acid (poly U) is irradiated with ultraviolet light. A molecule of water may add at the 4,-5 double bond of the uracil moieties as a result of irradiation and these may be reconverted to uracil by base-catalyzed dehydration. The other photoproduct formed is a uracil-uracil dimer, which reverts to uracil by reirradiation at lower wavelengths of ultraviolet light. The effects of irradiated poly U were studied iu the amino acid incorporating system in which dehydration and photoreversal of the irradiated poly U separated some of the ultraviolet effects. It was concluded that the water adduct is responsible for the coding transition of C14-phenylalanine to C14-serine, and the formation of dimer results in the loss of the incorporation of C14-phenylalanine, which is not replaced by any other amino acid.
- Research Organization:
- Brandeis Univ., Waltham, MA (United States)
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-17-040603
- OSTI ID:
- 4646231
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America, Vol. 50, Issue 4; Other Information: Orig. Receipt Date: 31-DEC-63; ISSN 0027-8424
- Publisher:
- National Academy of Sciences, Washington, DC (United States)
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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Related Subjects
AMINO ACIDS
BIOCHEMISTRY
CARBON 14
CHEMICAL REACTIONS
HETEROCYCLICS
IRRADIATION
KETONES
LABELLED COMPOUNDS
METABOLISM
ORGANIC ACIDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHENYLALANINE
POLYMERS
PYRIMIDINES
RADIATION CHEMISTRY
REACTION KINETICS
SERINE
TRACER TECHNIQUES
ULTRAVIOLET RADIATION
URACILS
URIDYLIC ACID
WATER