DISTRIBUTION OF RUBIDIUM-86 ACCUMULATED IN THE RABBIT LENS
Serial frontal sections were made of rabbit lenses after incubation in vitro in a simplified medium containing Rb/sup 86/ The earliest accumulation and highest accumulation ratios of Rb/sup 86/ were found in sections closest to the epithelium. In these anterior sections, values exceeded the medium concentration after 10-min incubation. The lowest concentrations were found deep in the nucleus of the lens. In the presence of ouabain (7 x 10/sup -6/M) the accumulation of Rb/sup 86/ showed a marked decrease (10 to 20% of normal) at all time intervals. The accumulation was particularly affected in areas closest to the lens epithelium. When the time course of accumulation was plotted for selected segments of the lens without ouabain, the accumulation in the area closest to epithelium was reasonably linear and very much more rapid than in areas farthest removed from epithelial structures. On the other hand, under the influence of ouabain ihhibition of the transport process, interior sections were remarkably similar in Rb/sup 86/ content. In a potassiumfree medium the 50% irhibitory concentration of ouabain was 7 x 10/sup -8/M, whereas in a medium containing 5 mM K/sup +/ it was closer to 1 x 10/sup -6/M. The most active areas demonstrate a sensitivity to ouabain inhibition which closely resembles that of the Na-- K-activated ATPase of rabbit lens epithelium. (BBB)
- Research Organization:
- Washington Univ., St. Louis
- NSA Number:
- NSA-17-038717
- OSTI ID:
- 4645556
- Journal Information:
- Investigative Ophthalmology (U.S.) Changed to Invest. Ophthalmol. Visual Sci., Vol. Vol: 1; Other Information: Orig. Receipt Date: 31-DEC-63
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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