THE EFFECT OF ACRIFLAVINE ON PHOTO-REVERSAL OF LETHAL AND MUTAGENIC DAMAGE PRODUCED IN BACTERIA BY ULTRAVIOLET LIGHT
Journal Article
·
· Proc. Natl. Acad. Sci. U.S.
ABS>The addition of acriflavine to the postirradiation plating medium has the following effects in strain WU36, a tyrosine-requiring substrain of E. coli B/r: it enhances both the lethal and mutagenic effects of uv, decreasing survival and increasing the yield of tyr+ mutations; it reduces the efficiency of photoreversal of induced prototrophs, increasing the amount of light required to accomplish maximal photoreversal about fourfold, while having no such effect on the photoreversal of uv killing; it reduces the efficiency of photoprotection against induced prototrophy, while having no such effect on photoprotection against uv killing. Mutations to prototrophy are reducible in frequency by pretreatment with visible light to the same extent as by posttreatment with visible light, although maximal photoprotection requires about four times as much light as maximal photoreversal. In contrast, very little photoprotection against uv killing is obtained in this strain. The results are interpreted as evidence that the lesions leading to photoreversible uv killing and those leading to photoreversible uvinduced prototrophy are different, either in their primary nature or in some unknown secondary feature. (auth)
- Research Organization:
- State Univ. of New York, Brooklyn
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-17-040600
- OSTI ID:
- 4618766
- Journal Information:
- Proc. Natl. Acad. Sci. U.S., Journal Name: Proc. Natl. Acad. Sci. U.S. Vol. Vol: 50
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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