Allozyme genotypic indicators of adaptive responses in contaminant-exposed populations: Consistency among fish species and contaminants

Conference · Sun Dec 31 04:00:00 EST 1995

OSTI ID:460476

Diamond, S A ^[1]; Guttman, S I; Oris, J T ^[2]

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1. Hagler Bailly Consulting, Boulder, CO (United States)
2. Miami Univ., Oxford, OH (United States)

Enzyme genotypic frequency shifts, or unique patterns have been suggested as useful indicators of long-term adaptive responses in contaminant-exposed populations. These genetic responses are also biologically relevant as they can result in loss of alleles and adaptive capacity. Enzyme genotypic data from several exposures were compared to determine whether genotype at specific enzyme loci, and at multiple loci, are consistently related to differential survival during toxicant exposure. Results of mosquitofish (Gambusia holbrooki) laboratory exposures to mercury and arsenic, genetic characterization of mosquitofish from a coal ash settling basin, and several acute and chronic exposures of fathead minnows (Pimephales promelas) to fluoranthene in the presence of UV light were compared. In mosquitofish exposures (laboratory and field) genotype at glucosephosphate isomerase (Gpi) loci were consistently related to time to death or apparent shifts in enzyme genotypic frequencies in field-exposed populations (P < 0.03 in all c{sup 2} comparisons). In addition, genotype-mortality relationships were consistent among laboratory mercury and arsenic exposures when multiple-locus genotypes were considered. When isocitrate dehydrogenase (Idh), Gpi, and malate dehydrogenase (Mdh) loci were combined the resulting multiple locus genotype was related to time to death (P < 0.03 in all c{sup 2} comparisons). Enzyme genotype at the Gpi locus was significantly related to time of mortality or enzyme frequency differences in 7 of 10 comparisons (P < 0.04 in all c{sup 2} comparisons).

• OSTI ID: 460476
• Report Number(s): CONF-961149--
• Country of Publication: United States
• Language: English

Related Subjects

01 COAL, LIGNITE, AND PEAT
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
ARSENIC
BIOLOGICAL ADAPTATION
BIOLOGICAL INDICATORS
BIOLOGICAL MARKERS
CONDENSED AROMATICS
ENVIRONMENTAL IMPACTS
FISHES
GENETIC EFFECTS
GENOTYPE
MERCURY
WATER POLLUTION