Site-specific variability in BTEX biodegradation under denitrifying conditions

Journal Article · Sat Mar 01 04:00:00 EST 1997 · Ground Water

DOI:https://doi.org/10.1111/j.1745-6584.1997.tb00087.x· OSTI ID:460459

Kao, C M ^[1]; Borden, R C ^[2]

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1. Geophex, Ltd., Raleigh, NC (United States)
2. North Carolina State Univ., Raleigh, NC (United States). Dept. of Civil Engineering

Laboratory microcosm experiments were conducted to evaluate the feasibility of benzene, toluene, ethylbenzene, m-xylene, and o-xylene (BTEX) biodegradation under denitrifying conditions. Nine different sources of inocula, including contaminated and uncontaminated soil cores from four different sites and activated sludge, were used to establish microcosms. BTEX was not degraded under denitrifying conditions in microcosms inoculated with aquifer material from Rocky Point and Traverse City. However, rapid depletion of glucose under denitrifying conditions was observed in microcosms containing Rocky Point aquifer material. TEX degradation was observed in microcosms containing Rocky Point aquifer material. TEX degradation was observed in microcosms containing aquifer material from Fort Bragg and Sleeping Bear Dunes and sewage sludge. Benzene was recalcitrant in all microcosms tested. The degradation of o-xylene ceased after toluene, ethylbenzene, and m-xylene were depleted in the Fort Bragg and sludge microcosms, but o-xylene continued to degrade in microcosms with contaminated Sleeping Bear Dunes soil. The most probable number (MPN) of denitrifiers in these nine different inocula were measured using a microtiter technique. There was no correlation between the MPN of denitrifiers and the TEX degradation rate under denitrifying conditions. Experimental results indicate that the degradation sequence and TEX degradation rate under denitrifying conditions may differ among sites. Results also indicate that denitrification alone may not be a suitable bioremediation technology for gasoline-contaminated aquifers because of the inability of denitrifiers to degrade benzene.

• Sponsoring Organization: Environmental Protection Agency, Washington, DC (United States)
• OSTI ID: 460459
• Journal Information: Ground Water, Journal Name: Ground Water Journal Issue: 2 Vol. 35; ISSN GRWAAP; ISSN 0017-467X
• Country of Publication: United States
• Language: English

Related Subjects

02 PETROLEUM
ALKYLATED AROMATICS
BENZENE
BIODEGRADATION
DENITRIFICATION
GROUND WATER
OIL SPILLS
REMEDIAL ACTION
TOLUENE
XYLENES