Toxicity of 17 {beta}-estradiol and dibutyl-n-phthalate to Japanese medaka (Oryzias latipes)
Conference
·
OSTI ID:458372
- Rutgers-The State Univ., Piscataway, NJ (United States)
Phthalate esters are ubiquitous environmental contaminants that are hypothesized to cause developmental toxicity in aquatic organisms via an estrogenic mechanism. Japanese medaka embryos and larvae provide an excellent model for the study of toxicant effects on embryonic development. The following groups were examined (N = 10--20): a non-treatment control, a vehicle control, 17 {beta}-estradiol and Dibutyl-n-phthalate, in individual glass vials. The medaka embryos were treated beginning at the blastula stage, for ten days. At day 10, embryos were changed into fresh rearing solution. The embryos were observed daily, until three days post-hatching, for toxic developmental effects. Exposure to 17 {beta}-estradiol caused urinary bladder lesions at the lowest doses tested. At concentrations {le} 3 {micro}M/0.82 ppm, 17 {beta}-estradiol caused inhibition of swim bladder inflation, pericardial edema, and marked cachexia. Dibutyl-n-phthalate caused pronounced enlargement of the urinary bladder. No other gross lesions were observed. Both 17 {beta}-estradiol and Dibutyl-n-phthalate caused effects on the urinary tract which will be characterized at the light microscopic level. The lesions observed in the embryo medaka following Dibutyl-n-phthalate exposure were at or below water solubility and are in agreement with previously reported toxic levels.
- OSTI ID:
- 458372
- Report Number(s):
- CONF-961149--
- Country of Publication:
- United States
- Language:
- English
Similar Records
Mechanistic studies of pericardial edema in early life stages (ELS) of medaka (Oryzias latipes)
Induced histopathologic alterations in early life stages (ELS) of medaka (Oryzias latipes)
Adduct formation of 7,12-dimethylbenz(a)anthracene in the embryo of the Japanese medaka (Oryzias latipes)
Conference
·
Sat Dec 30 23:00:00 EST 1995
·
OSTI ID:211968
Induced histopathologic alterations in early life stages (ELS) of medaka (Oryzias latipes)
Conference
·
Fri Dec 30 23:00:00 EST 1994
·
OSTI ID:40093
Adduct formation of 7,12-dimethylbenz(a)anthracene in the embryo of the Japanese medaka (Oryzias latipes)
Conference
·
Sat Dec 30 23:00:00 EST 1995
·
OSTI ID:372518