The effects of ionizing radiation on avian erythrocytes

Journal Article · Sat Jan 01 04:00:00 EST 1994 · Radiation Research

DOI:https://doi.org/10.2307/3578797· OSTI ID:45272

Lee, S W; Ducoff, H S ^[1]

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1. Univ. of Illinois, Urbana, IL (United States)

The effects of ionizing radiation were examined in terminally differentiated cells using nucleated chicken erythrocytes (RBCs) as the model. We used a hemolytic assay to score radiation damage to RBCs. Chicken RBCs received 0 to 100 Gy of radiation at dose rate of 10 Gy/min. Radiation-induced hemolysis occurred in a dose-dependent manner but not immediately after irradiation. Hemolysis became apparent at 24 h after treatment. A threshold for radiation dose response was observed. At doses below 30 Gy, hemolysis in irradiated samples was indistinguishable from that in nonirradiated controls. A total dose of 100 Gy was used for the split-dose experiments. The results showed that chicken RBCs were able to repair radiation damage and that the half-time for maximum recovery was approximately 30 min at 36{degrees}C. Recovery from {gamma} radiation was also affected by the interfraction temperature. 36 refs., 7 figs.

• Sponsoring Organization: USDOE
• OSTI ID: 45272
• Journal Information: Radiation Research, Journal Name: Radiation Research Journal Issue: 1 Vol. 137; ISSN RAREAE; ISSN 0033-7587
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL REPAIR
CHICKENS
DOSE-RESPONSE RELATIONSHIPS
ERYTHROCYTES
GAMMA RADIATION
HEMOLYSIS