Measuring bioavailable concentrations and partition coefficients using solid phase micro-extraction (SPME)
Conference
·
OSTI ID:452101
- Utrecht Univ. (Netherlands). Research Inst. of Toxicology
Bioavailability plays an important role in fate as well as effects of organic pollutants. Organic compounds may bind to humic acids, proteins or even to the wall of exposure vessels. Quantitative data for exposure concentration in in vivo or in vitro tests are therefore not always easy to interpret. Solid phase micro-extraction is an extraction technique which enables the measurement of freely dissolved concentrations of organic chemicals. This SPME method was used to measure protein binding, binding to microsomes, membrane-water partition coefficients and freely dissolved concentrations in in vitro tests of five different organic chemicals (aniline, nitrobenzene, 4-chloro-3-methylphenol, 4-n-pentylphenol and pentachlorobenzene). Exposure profiles (concentration time curves) in aqueous samples were determined for the SPME extraction. Based on these profiles, an exposure time was derived for each chemical separately. The bioavailable concentrations were measured in the presence of proteins, microsomes etc. in such a way that the SPME extraction causes a negligible depletion of the aqueous sample. The SPME method appears to be extremely useful for the purpose of determining freely dissolved concentrations in all types of matrices and also to determine partition coefficients.
- OSTI ID:
- 452101
- Report Number(s):
- CONF-961149--
- Country of Publication:
- United States
- Language:
- English
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