Application of the FETAX developmental toxicity assay for evaluating the success of bioremediation
Conference
·
OSTI ID:452097
- Oklahoma State Univ., Stillwater, OK (United States). Dept. of Zoology
- National Risk Management Lab., Ada, OK (United States)
A ten-year-old subsurface spill of JP-4 jet fuel at Eglin Air Force Base (FL) was remediated using nitrate application. The authors used the Frog Embryo Teratogenesis Assay-Xenopus (FETAX) before, during and after remediation to detect whether toxicity was altered. FETAX is a 96-hr whole embryo developmental toxicity assay used in ecotoxicology and in detecting mammalian developmental toxicants when an in vitro metabolic activation system is employed. FETAX developmental toxicity assays were performed on soil samples and extracts throughout remediation. They chose FETAX because development is a weak link in the life cycle of living organisms and indicative of chronic effects. Environmental chemistry was also performed to quantify TPH and BTEX concentrations in the soil. Preliminary FETAX experiments with pure JP-4 showed that JP-4 was more fetotoxic than teratogenic and metabolic activation did not greatly alter the development toxicity of pure JP-4. The newly developed technique of direct exposure was more successful with Eglin soil samples than aqueous extraction in this study because the direct exposure technique is better for hydrophobic materials such as JP-4. Using the direct exposure technique, there was a correlation noted between TPH and BTEX concentrations in the soil samples and developmental toxicity. A general decrease in developmental toxicity in both control and nitrate-treated plots was observed throughout the study. FETAX proved sensitive enough to detect this toxicity and useful in determining when sufficient bioremediation had occurred.
- OSTI ID:
- 452097
- Report Number(s):
- CONF-961149--
- Country of Publication:
- United States
- Language:
- English
Similar Records
FETAX interlaboratory validation study: Phase 2 testing
Integrated ecological hazard assessment of waste site soil extracts using FETAX and short-term fathead minnow teratogenesis assay
Developmental hazard assessment with FETAX: Aerobic metabolites in bacterial transformation of naphthalene
Journal Article
·
Sat Oct 01 00:00:00 EDT 1994
· Environmental Toxicology and Chemistry; (United States)
·
OSTI ID:6945571
Integrated ecological hazard assessment of waste site soil extracts using FETAX and short-term fathead minnow teratogenesis assay
Conference
·
Mon Dec 30 23:00:00 EST 1996
·
OSTI ID:524685
Developmental hazard assessment with FETAX: Aerobic metabolites in bacterial transformation of naphthalene
Journal Article
·
Mon May 01 00:00:00 EDT 1995
· Bulletin of Environmental Contamination and Toxicology
·
OSTI ID:48006