Diverse V{sub H} and V{sub {kappa}} genes encode antibodies to Pseudomonas aeruginosa LPS

Journal Article · Sun Oct 15 04:00:00 EDT 1995 · Journal of Immunology

OSTI ID:447017

Emara, M G; Tout, N L; Kaushik, A; Lam, S ^[1]

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1. Univ. of Guelph, Ontario (Canada)

The molecular nature of the murine Ab response to Pseudomonas aeruginosa LPS was examined using a panel of 10 well-defined anti-LPS mAbs. Abs to P. aeruginosa LPS are encoded by diverse V-genes, with at least five V{sub H} and four V{sub {kappa}} gene families represented in these Abs. The Abs that bind to hydrophilic O-polysaccharide side chains of B-band LPS and A-band LPS are encoded by V{sub H}J558, SM7, and J606 gene families, while Abs to hydrophobic core and lipid A regions are encoded by X24, SM7, and Q52 gene families. All active J{sub H} and only two J{sub {kappa}} (J{sub {kappa}}2 and J{sub {kappa}}5) germ-line genes are utilized in the anti-LPS Abs examined. Four of six anti-P. aeruginosa mAbs used diversity genes of the DPS2 gene family. Interestingly, J{sub H}1 and J{sub H}2 use was observed in three mAbs that reacted with hyprophilic LPS epitopes (O-polysaccharide, A-band LPS), whereas J{sub H}3 and J{sub H}4 use was observed in three mAbs that bound to the more hydrophobic regions of LPS (core, lipid A). Point mutations were observed in framework and complementarity-determining regions (CDRs) of V{sub H} and V{sub L} genes, suggesting an Ag-driven maturation process in response to P. aeruginosa LPS. Mutations occurred in all heavy chain CDRs, as well as in CDR1 and CDR3 of the light chain, indicating an important role of these regions in binding to LPS. These data suggest that diverse V{sub H} and V{sub {kappa}} genes encode Abs to LPS from P. aeruginosa. 52 refs., 6 figs., 1 tab.

• OSTI ID: 447017
• Journal Information: Journal of Immunology, Journal Name: Journal of Immunology Journal Issue: 8 Vol. 155; ISSN JOIMA3; ISSN 0022-1767
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
ANTIBODIES
DNA SEQUENCING
DNA-CLONING
GENE MUTATIONS
GENES
INFECTIOUS DISEASES
MICE
POLYMERASE CHAIN REACTION
PROBES
PSEUDOMONAS
STRUCTURE-ACTIVITY RELATIONSHIPS
THERAPY