Cloning and gene mapping of the mouse homologue of the CBFA2T1 gene associated with human acute myeloid leukemia
- Saitama Cancer Center Research Inst. (Japan); and others
The human CBFA2T1 (also known as MTG8) gene, on chromosome 8, has been identified through its involvement in the t(8;21) chromosomal translocation, frequently found in acute myeloid leukemia. We report here the isolation and characterization of the mouse homologue of the CBFA2T1 gene, Cbfa2t1h. Nucleotide sequence analysis of Cbfa2t1h cDNA clones revealed an open reading frame encoding a protein of 577 amino acids with an extremely high degree of amino acid identity (99.3%) to the human protein. The nucleotide sequence is also highly conserved between mouse and human in the 5{prime}- and 3{prime}-untranslated regions (87.0, 92.0, and 93.7% identities for 5{prime}-untranslated, coding, 3{prime}-untranslated regions, respectively). The 3{prime}-untranslated region of Cbfa2t1h contains a (CA){sub n} dinucleotide repeat, and the polymerase chain reaction amplification of the (CA){sub n} repeat region revealed fragment length polymorphism among mouse strains. Using this polymorphism, we have mapped Cbfa2t1h to mouse chromosome 4 close to the centromere using SMXA recombinant inbred strains and 106 intersubspecific backcross progenies of the (DBA/2 {times} Mae) {times} Mae cross. The chromosomal location was also confirmed by fluorescence in situ hybridization. 23 refs., 3 figs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 443876
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 3 Vol. 29; ISSN GNMCEP; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BASIC STUDIES
BIOLOGICAL MARKERS
CHIMERAS
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
FLUORESCENCE
GENE REGULATION
GENES
GENETIC MAPPING
HUMAN CHROMOSOME 21
HUMAN CHROMOSOME 8
HUMAN POPULATIONS
HYBRIDIZATION
MICE
MYELOID LEUKEMIA
NEOPLASMS
NUCLEOTIDES
POLYMERASE CHAIN REACTION