Partial structure of the mouse glucokinase gene

Journal Article · Tue Oct 10 04:00:00 EDT 1995 · Genomics

DOI:https://doi.org/10.1006/geno.1995.9942· OSTI ID:443875

Ishimura-Oka, Kazumi; Chu, Mei-Jin; Sullivan, M; Oka, Kazuhiro ^[1]

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1. Baylor College of Medicine, Houston, TX (United States)

A complementary DNA for glucokinase (GK) was cloned from mouse liver total RNA by a combination of the polymerase chain reaction (PCR) and mouse liver cDNA library screening. Liver- and {beta}-cell-specific exons 1 were isolated by PCR using mouse and rat genomic DNAs. These clones were then used to screen a mouse genomic library; three genomic clones were isolated and characterized. The mouse GK gene spans over 20 kb, containing 11 exons including a liver- or {beta}-cell-specific exon 1, which encodes a tissue-specific 15-aa peptide at the N-terminus of the protein. Both types of GK contain 465 amino acid residues. The predicted amino acid sequence of mouse {beta}-cell-specific GK showed 98 and 96% identity to the rat and human enzymes, respectively; the corresponding values are 98 and 95% respectively, for the liver-specific GK. Several transcription factor-binding consensus sequences are identified in the 5{prime} flanking region of the mouse GK gene. 21 refs., 1 fig.

• Sponsoring Organization: USDOE
• OSTI ID: 443875
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 3 Vol. 29; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
AMINO ACID SEQUENCE
BIOLOGICAL EVOLUTION
DNA SEQUENCING
DNA-CLONING
GENE REGULATION
GENETIC MAPPING
HEXOKINASE
HUMAN POPULATIONS
LIVER CELLS
MICE
POLYMERASE CHAIN REACTION
RATS
STRUCTURE-ACTIVITY RELATIONSHIPS
TRANSCRIPTION