A 6-Mb yeast artificial chromosome contig and long-range physical map encompassing the region on chromosome 12q15 frequently rearranged in a variety of benign solid tumors

Journal Article · Tue Oct 10 04:00:00 EDT 1995 · Genomics

DOI:https://doi.org/10.1006/geno.1995.9952· OSTI ID:443865

Schoenmakers, E F.P.M.; Geurts, J M.W.; Kools, P F.J.; Mols, R ^[1]

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1. Univ. of Leuven (Belgium); and others

Cytogenetic analysis of a variety of benign solid tumors, among which uterine leiomyoma, lipoma, pleomorphic salivary gland adenoma, and pulmonary chondroid hamartoma, has indicated that these tumors often display chromosome breakpoints in region q13-q15 of chromosome 12. In previous studies, we have reported that these breakpoints map between locus D12S8 and the CHOP gene, the latter of which has been shown to be consistently rearranged in myxoid liposarcomas with t(12;16)(q13;p11). Here, we report directional chromosome walking studies starting from D12S8 and resulting in the construction of a YAC contig of about 6 Mb. This YAC contig, whose orientation on chromosome 12 was determined by double-color fluorescence in situ hybridization (FISH) analysis, has at least double coverage and consists of 75 overlapping YAC clones, all isolated from CEPH YAC libraries. Their insert sizes were estimated by contour-clamped homogeneous electric field (CHEF) gel electrophoresis. On the basis of YAC end-derived DNA markers and sequence-tagged sites (STSs), with an average spacing of approximately 70 kb, as well as restriction enzyme analysis, a long-range physical map was established for the 6-Mb DNA region of chromosome 12 covered by the YAC contig. Within the YAC contig, the relative positions of various known genes, an expressed sequence-tagged site, and a number of CEPH/Genethon polymorphic markers were determined. The latter data allow full integration of our mapping data with those obtained by CEPH/Genethon as well as those reported at the Second International Workshop on Human Chromosome 12 Mapping. Finally, this YAC contig constitutes the basis for the construction of a transcriptional map of this region and is likely to facilitate identification of genes involved in the formation of various benign solid tumor types. 56 refs., 1 fig., 2 tabs.

• Sponsoring Organization: USDOE
• OSTI ID: 443865
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 3 Vol. 29; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
BIOLOGICAL MARKERS
CHIMERAS
CHROMOSOMAL ABERRATIONS
CONTIGS
DESIGN
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
ELECTROPHORESIS
FLUORESCENCE
GENE MUTATIONS
GENES
GENETIC MAPPING
HUMAN CHROMOSOME 12
HUMAN CHROMOSOME 16
NEOPLASMS
POLYMERASE CHAIN REACTION
TRANSCRIPTION