Isolation of the mouse homologue of BRCA1 and genetic mapping to mouse chromosome 11

Journal Article · Tue Oct 10 04:00:00 EDT 1995 · Genomics

DOI:https://doi.org/10.1006/geno.1995.9963· OSTI ID:443854

Bennett, L M; Haugen-Strano, A; Cochran, C ^[1]

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1. National Inst. of Environmental Health Sciences, Research Triangle Park, NC (United States); and others

The BRCA1 gene is in large part responsible for hereditary human breast and ovarian cancer. Here we report the isolation of the murine Brca1 homologue cDNA clones. In addition, we identified genomic P1 clones that contain most, if not all, of the mouse Brca1 locus. DNA sequence analysis revealed that the mouse and human coding regions are 75% identical at the nucleotide level while the predicted amino acid identity is only 58%. A DNA sequence variant in the Brcal locus was identified and used to map this gene on a (Mus m. musculus Czech II x C57BL/KsJ)F1 x C57BL/KsJ intersubspecific backcross to distal mouse chromosome 11. The mapping of this gene to a region highly syntenic with human chromosome 17, coupled with Southern and Northern analyses, confirms that we isolated the murine Brcal homologue rather than a related RING finger gene. The isolation of the mouse Brca1 homologue will facilitate the creation of mouse models for germline BRCA1 defects. 12 refs., 3 figs.

• Sponsoring Organization: USDOE
• OSTI ID: 443854
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 3 Vol. 29; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
AMINO ACID SEQUENCE
BIOLOGICAL MODELS
CHROMOSOMES
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
GENE MUTATIONS
GENES
GENETIC MAPPING
HEREDITARY DISEASES
HUMAN CHROMOSOME 17
MAMMARY GLANDS
MICE
NEOPLASMS
OVARIES
PATIENTS
POLYMERASE CHAIN REACTION
TRANSCRIPTION