Influence of host cell repair genes on the survival of phages T$sub 3$, T$sub 7$, and T$sub 5$ B after treatment with 8-methoxypsoralen plus long wavelength uv light

Journal Article · Mon Jan 01 04:00:00 EST 1973 · Stud. Biophys., v. 36-37, pp. 81-96

OSTI ID:4423870

Godsell, A; Collis, T; Clarke, C

From international conference on the bases of the biological effects of ultraviolet radiation; Brno, Czechoslovakia (2 Oct 1972). Following irradiation of phages T/sub 3/, T/sub 7/, and the HCR/sup -/ suspec table type of T/sub 5/ (T/ sub 5/ B) with long wavelength uv light, in the presence of 8- methoxypsoralen, survival was assayed on a variety of E. coli host strains differing in known dark- repair functions. In some experiments survival was assayed in the presence or absence of caffeine, at a concentration of 500 mu g/ml, to effect phenotypic blockage of the HCR, excision repair, system. Results show that in the case of phages T/sub 3/ and T/sub 7/ the photosensitized damage is repaired by excision repair, and to a lesser extent by the host cell rec gene system. No influence of exr or pol A gene functions on phage survival was found. In the case of phage T/ sub 5/ B damage by long wavelength UV + 8-methoxysoralen is repaired by the HCR (excision repair) and pol A systems, but no effect of exr or rec host genes on phage survival was found. (auth)

• Research Organization: Univ. of East Anglia, Norwich, Eng.
• NSA Number: NSA-29-000565
• OSTI ID: 4423870
• Journal Information: Stud. Biophys., v. 36-37, pp. 81-96, Journal Name: Stud. Biophys., v. 36-37, pp. 81-96; ISSN STBIB
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

*BACTERIOPHAGES-- BIOLOGICAL RADIATION EFFECTS
*CAFFEINE-- RADIOSENSITIVITY EFFECTS
*GENES-- RADIOSENSITIVITY EFFECTS
*PSORALEN-- RADIOSENSITIVITY EFFECTS
BIOLOGICAL REPAIR
N48310* --Life Sciences--Radiation Effects on Microorganisms- -Basic Studies
PHOTON BEAMS
RESPONSE MODIFYING FACTORS
SURVIVAL TIME
ULTRAVIOLET RADIATION