Histoincompatibility recognition reaction by irradiated or mitomycin- treated parental cells against hybrid spleen cells produces hybrid cell proliferation and illusion of reaction of hybrid against parent

Journal Article · Sat Dec 01 04:00:00 EST 1973 · J. Immunol., v. 111, no. 6, pp. 1639-1652

OSTI ID:4368955

Kennedy, J C; Ekpaha-Mensah, J A

The addition of heavily irradiated or mitomycin-treated spleen cells to cultures containing syngeneic spleen cells and foreign erythrocytes may stimulate to some extent the hemolytic plaqueforming cell responses of these cultures, but the addition of semi-allogeneic irradiated or mitomycin-treated spleen cells may cause much greater stimulation. This semi-allogeneic stimulation is completely reciprocal; the plaque-forming cell responses of /sub 1/ hybrid spleen cell cultures often are stimulated as much after the addition of non-proliferating parental cells as are the responses of parental spleen cell cultures after the addition of non-proliferating F/sub 1/ hybrid cells. The possibility that an in vitro manifestation of hybrid resistance might be involved was ruled out. It was found that irradiated or mitomycin-treated cells, although unable to proliferate, were still able to initiate a recognition reaction against histoincompatible cells, releasing in the process a soluble factor that stimulated proliferation of cells of the antibody-forming series. The significance of this finding for one- way mixed lymphocyte reactions is discussed. (auth)

• Research Organization: Queen's Univ., Kingston, Ont.
• NSA Number: NSA-29-007906
• OSTI ID: 4368955
• Journal Information: J. Immunol., v. 111, no. 6, pp. 1639-1652, Journal Name: J. Immunol., v. 111, no. 6, pp. 1639-1652; ISSN JOIMA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

*SOMATIC CELLS-- BIOLOGICAL RADIATION EFFECTS
*SPLEEN CELLS-- BIOLOGICAL RADIATION EFFECTS
CELL CULTURES
CELL PROLIFERATION
GAMMA RADIATION
IMMUNITY
IRRADIATION
MITOMYCIN
N48210* --Life Sciences--Radiation Effects on Cells-- External Source
PHOTON BEAMS SPLEEN CELLS