Strand breaks and 5' end-groups in DNA of irradiated thymocytes

Journal Article · Mon Oct 01 04:00:00 EDT 1973 · Int. J. Radiat. Biol., v. 24, no. 4, pp. 397-404

DOI:https://doi.org/10.1080/09553007314551251· OSTI ID:4356808

Coquerelle, T; Bopp, A; Kessler, B; Hagen, U

Rat thymocytes suspended in Hank's solution were irradiatedwith 10 MeV electrons up to 6 Mrads in air at 0 deg C. Single- and double-strand breaks in the DNA were determined in the analytical centrifuge. 5' end-groups were characterized by specific phosphorylation with polynucleotide kinase before and after phosphatase treatment. The single-strand breaks were formed with an efficiency value of l09 plus or minus 6 eV per break and the double-strand breaks with 1940 plus or minus 110 eV. 14% of the 5' terminals carried a hydroxyl group and 20% a phosphate group. Polynucleotide kinase did not react with the other 66% 5' end-groups. For comparison, dry calf-thymus DNA was irradiated in vacuo. Under these conditions, 22% of the 5' terminals carry a phosphate end group, whereas no 5'OH groups could be found. (auth)

• Research Organization: Kernforschungszentrum, Karlsruhe, Ger.
• NSA Number: NSA-29-015999
• OSTI ID: 4356808
• Journal Information: Int. J. Radiat. Biol., v. 24, no. 4, pp. 397-404, Journal Name: Int. J. Radiat. Biol., v. 24, no. 4, pp. 397-404; ISSN IJRBA
• Country of Publication: United Kingdom
• Language: English

Related Subjects

*DNA-- STRAND BREAKS
*STRAND BREAKS-- RADIOINDUCTION
BIOLOGICAL RADIATION EFFECTS
CALVES
CENTRIFUGATION
COMPARATIVE EVALUATIONS
ELECTRON BEAMS
ELECTRONS
EV RANGE 100-1000
KEV RANGE 01-10
MEDIUM TEMPERATURE
MEV RANGE 01-10
MOLECULAR STRUCTURE
N48520* --Life Sciences--Radiation Effects on Animals-- Vertebrates
PHOSPHATASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
RATS
SUSPENSIONS
THYMOCYTES
THYMUS