Tumor-Host Cell Hybrids in Radiochimeras

Journal Article · Tue Jan 01 04:00:00 EST 1974 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.71.1.148· OSTI ID:4351528

Wiener, F.; Fenyo, E. M.; Klein, G.

F₁ hybrid mice syngeneic or semiallogeneic with respect to the relevant tunnor were lethally irradiated and then reconstituted with hemopoietic cells from strain CBAT6T6 mice. After chimerism had been established, the animals were inoculated with solid or ascites tumors. Tumor-host cell hybrids were selected from enzyme-deficient solid tunnors by explanting the tumor cell suspension into hypoxanthine-amethopterinhymidine containing medium. The selection of hybrid cells from ascites tumors was achieved by exploiting the difference between the ascites tumor cells and hybrid cells in their ability to adhere to the surface of culture vessels. T6T6 chromosomal and H-2 antigenic markers served to distinguish between the hemopoietic cells derived from the donor graft and the cells of the host. All solid tunnors tested fused with cells of the irradiated host, whereas ascites tumors fused with repopulating cells of hemopoietic origin.

• Research Organization: Karolinska Inst., Stockholm
• Sponsoring Organization: USDOE
• NSA Number: NSA-29-021205
• OSTI ID: 4351528
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 1 Vol. 71; ISSN 0027-8424
• Publisher: National Academy of Sciences, Washington, DC (United States)
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

*EHRLICH ASCITES TUMOR
*RADIATION CHIMERAS
*TUMOR CELLS-- HYBRIDIZATION
62 RADIOLOGY AND NUCLEAR MEDICINE
BLOOD CELLS
LETHAL IRRADIATION
MICE
NEOPLASMS