Post-irradiation effects of photoreactivating light and caffeine on cultured marsupial cells exposed to ultraviolet light

Journal Article · Thu Nov 01 00:00:00 EST 1973 · Photochem. Photobiol., v. 18, no. 5, pp. 433-436

DOI:https://doi.org/10.1111/j.1751-1097.1973.tb06445.x· OSTI ID:4336803

Todd, P; Schroy, C B; Lebed, M R

Cells from the kidney of the rat kangeroo (Potorous tidactylus) were used for in vitro studies of the postirradiation effects of photoreactivating light and caffeine. Survival curves indicated that the effect of post-uv exposure (100 ergs mm/sup -2/) to photoreactivating light for 30 min was dose modifying; the ratio of doses leading to any level of damage on the two curves was nearly constant with a value of approximately 2.3 plus or minus 0.2. Potorous cells were found to be resistant to the toxic effects of caffeine up to 4.0 mM with only moderate cell killing but long growth delay. Survival curves are presented for cells post-irradiation incubated in 1.0 mM, 1.5 mM, and 4.0 mM caffeine. Incubation for up to 60h showed no subtoxic level that significantly modifed cell survival. It is suggested that these in vitro studies indicate that the presence of photoreactivating enzyme discussed by Cooke, J. S., and Regan, J. D., (Nature 223, 1066-1067, 1969) phylogenetically parallels the presence of photoreactivation of lethal damage. (UK)

• Research Organization: Pennsylvania State Univ., University Park
• NSA Number: NSA-29-016015
• OSTI ID: 4336803
• Journal Information: Photochem. Photobiol., v. 18, no. 5, pp. 433-436, Other Information: Orig. Receipt Date: 30-JUN-74; Bib. Info. Source: UK (United Kingdom (sent to DOE from))
• Country of Publication: United Kingdom
• Language: English

Related Subjects

N48520* -Life Sciences-Radiation Effects on Animals- Vertebrates
*ANIMAL CELLS- BIOLOGICAL RADIATION EFFECTS
*CAFFEINE- RADIOSENSITIVITY EFFECTS
BIOLOGICAL REPAIR
DILUTION
DOSE-RESPONSE RELATIONSHIPS
IN VITRO
LETHAL IRRADIATION
MARSUPIALS
PHOTON BEAMS
RADIOSENSITIVITY
RESPONSE MODIFYING FACTORS
SURVIVAL CURVES
TIME DEPENDENCE
TOXICITY
ULTRAVIOLET RADIATION
VISIBLE RADIATION