THE UNRELIABILITY OF THE INTRA-PERITONEAL INJECTION ROUTE AND THE VALIDITY OF EXPERIMENTAL OBSERVATIONS

Technical Report · Wed Jan 01 04:00:00 EST 1958

OSTI ID:4335103

Baxter, R C; Tuttle, L W

The intra-peritoneal injection method can be unreliable and lead to gross misinterpretation of experimental results which depend upon the adequacy of a single injection of radioisotope, drug or other pharmacologic agent. A method was devised by which the establishment of a given body burden of bone seeking radioisotope, Strontium-90 and Yttrium-90, in small laboratory animals was monitored daily by bremsstrahlen counts during the administration period. The use of this method permits the immediate detection of any aberrant individuals so that they may be given additional treatments to insure that all animals in the experiment have the same initial body burden. This equalization of body burden is of importance when long-term effects such as life span shortening and bone tumor incidence in relation to radiation dosage are of primary interest. In our series of 360 injections into 40 rats, partial or complete mis-injection of the isotope into the intestinal tract occurred on an average of 10% of the time and ranged from 0 to 17.5%. (auth)

• Research Organization: Rochester, N.Y. Univ. Atomic Energy Project
• NSA Number: NSA-12-007647
• OSTI ID: 4335103
• Report Number(s): UR-516
• Country of Publication: United States
• Language: English

Related Subjects

BIOLOGY AND MEDICINE
BONES
BREMSSTRAHLUNG
CARCINOGENESIS
DETECTION
DRUGS
INTESTINE
LABORATORY EQUIPMENT
MONITORING
RADIATION DOSES
RATS
STRONTIUM 90
SURVIVAL TIME
TUMORS
USES
YTTRIUM 90