Effect of uv-light, caffeine, and nalidixic acid on division ability of uv- sensitive filament-forming mutants of Salmonella typhimurium

Journal Article · Tue Jan 01 04:00:00 EST 1974 · Genetika, v. 10, no. 1, pp. 123-129

OSTI ID:4332536

Pokrovskii, V N; Guseva, O A

The data presented have shown that the addition of caffeine to the plating medium used to assay postirradiation viability of uv-sensitive filament- forming mutants of Salmonella typhimurium resulted in decreased survival. Liquid holding recovery of the mutants is inhibited by caffeine. The uv-sensitive filament-forming mutants of S. typhimurium are very sensitive to nalidixic acid. Incubation in the medium containing nalidixic acid induced long filament formation in mutants. Microscopic observation of uv-irradiated mutant P20 cells indicated that filamentous cells resume cell division and possessed the colony- forming ability. The observed identity of the uv-survival curves of non- lysogenic and lysogenic cells is due to the fact that uv-irradiated lysogenic cells divide before lysis, yielding both an infective center and a surviving colony. (auth)

• Research Organization: Inst. of Epidemiology and Microbiology, Moscow
• NSA Number: NSA-29-029697
• OSTI ID: 4332536
• Journal Information: Genetika, v. 10, no. 1, pp. 123-129, Journal Name: Genetika, v. 10, no. 1, pp. 123-129; ISSN GNKAA
• Country of Publication: Country unknown/Code not available
• Language: Russian

Related Subjects

*CAFFEINE-- RADIOSENSITIVITY EFFECTS
*ORGANIC ACIDS-- RADIOSENSITIVITY EFFECTS
*SALMONELLA TYPHIMURIUM-- BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
MUTANTS
N48310* --Life Sciences--Radiation Effects on Microorganisms- -Basic Studies
SURVIVAL TIME
ULTRAVIOLET RADIATION