Quantitative image analysis of HIV-1 infection in lymphoid tissue

Journal Article · Fri Nov 08 04:00:00 EST 1996 · Science

DOI:https://doi.org/10.1126/science.274.5289.985· OSTI ID:433222

Haase, A T; Zupancic, M; Cavert, W ^[1]

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1. Univ. of Minnesota Medical School, Minneapolis, MN (United States); and others

Tracking human immunodeficiency virus-type 1 (HIV-1) infection at the cellular level in tissue reservoirs provides opportunities to better understand the pathogenesis of infection and to rationally design and monitor therapy. A quantitative technique was developed to determine viral burden in two important cellular compartments in lymphoid developed to determine viral burden in two important cellular compartments in lymphoid tissues. Image analysis and in situ hybridization were combined to show that in the presymptomatic stages of infection there is a large, relatively stable pool of virions on the surfaces of follicular dendritic cells and a smaller pool of productivity infected cells. Despite evidence of constraints on HIV-1 replication in the infected cell population in lymphoid tissues, estimates of the numbers of these cells and the virus they could produce are consistent with the quantities of virus that have been detected in the bloodstream. The cellular sources of virus production and storage in lymphoid tissues can now be studied with this approach over the course of infection and treatment. 22 refs., 2 figs., 2 tabs.

• OSTI ID: 433222
• Journal Information: Science, Journal Name: Science Journal Issue: 5289 Vol. 274; ISSN SCIEAS; ISSN 0036-8075
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
AIDS VIRUS
IMAGE PROCESSING
IMAGES
LYMPHATIC SYSTEM
PATHOGENESIS
VIRAL DISEASES