Interaction of bacterial and lambda phage recombination systems in the x- ray sensitivity of Escherichia coli K-12
Journal Article
·
· Proc. Nat. Acad. Sci. U. S. A., v. 71, no. 2, pp. 503-506
E. coli cells lysogenic for the thermoinducible prophage lambda c1857 can be transiently induced by a brief heat treatment. Although this treatment does not kill the cells, some lambda products normally formed during vegetative phage development are made that can alter the response of host cells to x irradiation by causing an increase in radioresistance. This increased resistance is particularly striking in the recombination-deficient recB/sup -/ strain; which is normally much more radiosensitive than its recB/sup +/ parent. After pulse- heating at 42 deg C, the survival curve of E. coli recB/sup -/ lysogeuized with lambda c1857 does not differ from that of the wild-type strain. Since lambda red mutants do not increase the radioresistance of recB/sup -/ strains, both lambda red gene products, lambda exonuclease and BETA -protein, are required to compensate for the missing recB product. Furthermore, phage-induced radioresistance also occurs in recB/sup +/ lysogens even when they carry lambda red/sup -/, but not when the lambda prophage is gam/sup -/. Thus, in wild-type cells, phage-induced radioresistance requires some interaction between the bacterial recB gene product (exonuclease V) and the phage gamma -protein. (auth)
- Research Organization:
- Yale Univ., New Haven
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-29-024450
- OSTI ID:
- 4331880
- Journal Information:
- Proc. Nat. Acad. Sci. U. S. A., v. 71, no. 2, pp. 503-506, Journal Name: Proc. Nat. Acad. Sci. U. S. A., v. 71, no. 2, pp. 503-506; ISSN PNASA
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
Similar Records
Recombination of bacteriophage phiX174 by the red function of bacteriophage lambda. [Uv radiation]
Molecular mechanisms of mutagenesis and carcinogenesis. Final report, November 1, 1978-October 31, 1979
Induced radioresistance in four strains of Escherichia coli, two with lambda lysogens
Journal Article
·
Wed Jan 31 23:00:00 EST 1979
· J. Virol.; (United States)
·
OSTI ID:6242554
Molecular mechanisms of mutagenesis and carcinogenesis. Final report, November 1, 1978-October 31, 1979
Technical Report
·
Mon Mar 31 23:00:00 EST 1980
·
OSTI ID:5372652
Induced radioresistance in four strains of Escherichia coli, two with lambda lysogens
Journal Article
·
Thu Jun 01 00:00:00 EDT 1978
· Biophys. J.; (United States)
·
OSTI ID:6845057