EFFECTS OF RADIOCOBALT IRRADIATION OF RABBIT SPERMATOZOA IN VITRO ON FERTILIZATION AND EARLY DEVELOPMENT
ABS>Rabbit semen irradiated with gamma radiatlon from a Co-60 source in vitro at 90 r to 65,000 r was inseminated artificially to females. The ova were examined at pronuclear, segmentation, blastocyst, and into fetal stages. The motility of spermatozoa was damaged only at very high doses of irradiation, 32,200 to 65,000 r. Though incapacity of sperm to reach the site of fertilization may occur at these dose levels, the fertilizing capaeity per se appears to be undamaged. The segmentation of fertilized ova was very much disturbed. The disturbance is in proportion to the dose level and the higher the dose level the earlier the failure of segmentation. With irradiation of sperm at 90 r, the proportion of morphologically normal fetuses is not significantly lower than after normal mating; following irradiation of sperm at 600 r the formation of normal blastocysts was impaired but once a normal blastocyst is formed implantation and development are assured; following irradiation of sperm at 6,500 r, no normal blastocyst is formed. Delayed penetration of sperm, a high proportion of polyspermic ova, haploidy and triploidy of ova, and delayed syngamy were not observed following the irradiation of sperm at 32,200 to 65,000 r. Fragmentation of female and male pronuclei, an irregularity of nucleus formation at segmentation, and one possible gynogenetic ovum out of 32 ova were observed. (auth)
- Research Organization:
- Worchester Foundation for Experimental Biology, Shrewsbury; Boston Univ., Mass.
- NSA Number:
- NSA-12-011237
- OSTI ID:
- 4322602
- Journal Information:
- Anat. Record, Vol. Vol: 129, No. 2, Issue 2; Other Information: Orig. Receipt Date: 31-DEC-58; ISSN 0003--276X
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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