Chemical and biological techniques for identifying irradiated foods and food constituents-carbonyls and 2-deoxygluconic acid
From international colloquium: the identification of irradiated foodstuffs; Karlsruhe, Germany (24 Oct 1973). New microbiological and chemical procedures are described for the detection, concentration, and identification of compounds produced in irradiated foods. The microbiological method employs an equation, by which inhibition of cell growth is expressed in a simple, economic, and consistent manner for identification of different classes of growth inhibitors using a single growth index, namely, concentration of substance at which time needed for log phase cells to double is ten times the controls. The chemical procedure permits carbonyl compounds to be spectrophotometrically determined in the presence of carbohydrates without prior separation. The G(carbonyl) yields have been measured in irradiated hexoses and disaccharide solutions in the presence and absence of OH scavengers (KI and formate) and e/sub aq/ scavengers (N/sub 2/O). It has been demonstrated that the cytotoxic carbonyls produced upon carbohydrate irradiation are dicarbonyl sugars which convert to alpha , BETA -unsaturated carbonyl sugars by enolization or dehydration upon heating. Carbonyl yields in various irradiated foods are reported (e.g., strawberries: G = 2.2 and beef, G = 0.8). Both the carbonyl and microbiological techniques are uniquely applicable to the identification of non- volative compounds produced in irradiated foods. By anion exchange, we can rapidly separate enolizable carbonyls and 2-deoxygluconic acid from neutrnl sugars and obtain concentration factors of 50-fold and greater, thus enabling us to differentiate irradiated from nonirradiated foods at low radiation doses (approximately 1 Krad). (GE)
- Research Organization:
- Univ. of Pittsburgh
- NSA Number:
- NSA-29-018187
- OSTI ID:
- 4319733
- Resource Relation:
- Conference: International colloquium: the identification of irradiated foodstuffs, Karlsruhe, Germany, 24 Oct 1973; Other Information: Orig. Receipt Date: 30-JUN-74; Bib. Info. Source: GE (GE)
- Country of Publication:
- Germany
- Language:
- English
Similar Records
Identification of carbohydrate-containing foodstuffs by analysis of radiation specific substances
Identification of irradiated strawberries
Related Subjects
N47321 -Isotope & Radiation Source Technology-Radiation Sources-Industrial Uses-Food Processing
N48320 -Life Sciences-Radiation Effects on Microorganisms-Food Preservation
*DISACCHARIDES- CHEMICAL RADIATION EFFECTS
*FOOD- RADIOPRESERVATION
*HEXOSES- CHEMICAL RADIATION EFFECTS
G VALUE
GROWTH
INHIBITION
MICROORGANISMS
SPECTROPHOTOMETRY