Development of a radioligand tissue receptor assay for human follicle- stimulating hormone

Journal Article · Fri Feb 01 04:00:00 EDT 1974 · Endocrinology, v. 94, no. 2, pp. 483-491

OSTI ID:4318705

Reichert, Jr, L E; Bhalla, V K

A rat testes tubule receptor preparation has been characterized with respect to its usefulness for measurement of FSH activity in fractions derived from human pituitary glands. Highly purified human FSH, iodinated by a modification of the chloramine-T procedure, was employed as the radioligand. A series of human gonadotropin fractions were assayed in the FSH radioligandreceptor assay (RLA) and also in the Steelman-Pohley hCGaugmented rat ovarian weight gain assay (S-P assay), using LER-907 (20 IU/mg) as the assay reference preparation in each instance. The index of discrimination (ID) for a series of hFSH fractions ranging in potency from 53 IU/mg to 3608 IU/mg was 1.05. Highly purified hLH, hTSH and hCG showed /sup 125/I-hFSH uptake inhibition activity ranging from 0.9 to 3.6% that of highly purified hFSH. Significant uptake inhibition of labeled hFSH could be obtained with 2.5 ng of pure hFSH, while no uptake inhibition was seen with 10,000 ng of human growth hormone, human prolactin, ovine prolactin, ACTH and human serum albumin. AsialohFSH had a potency in the RLA 680 times greater than in the S-P assay, indicating that as with hLH and hCG, sialic acid is not required for receptor binding activity of hFSH. The usefulness of the RLA in following the combination of subunits of hFSH was also demonstrated. (auth)

• Research Organization: Emory Univ., Atlanta
• NSA Number: NSA-29-018744
• OSTI ID: 4318705
• Journal Information: Endocrinology, v. 94, no. 2, pp. 483-491, Journal Name: Endocrinology, v. 94, no. 2, pp. 483-491; ISSN ENDOA
• Country of Publication: Country unknown/Code not available
• Language: English

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HORMONES
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