Free-living amoebae used to isolate consortia capable of degrading trichloroethylene
- Oak Ridge National Lab., TN (United States)
- Univ. of Tennessee, Knoxville, TN (United States)
The interaction of protozoa with prokaryotes is well documented. These interactions can be either ecto- or endosymbiotic. An example of photosynthetic symbiosis is the well-defined interaction between paramecium and entrapped Chlorella. Paramecium can also form symbiotic relationships with gram-negative heterotrophic bacteria. Jeon has described an interaction between amoebae and engulfed bacteria, that eventuated into a dependency of the amoebae on the presence of the engulfed bacterium. Free-living amoebae and tetrahymena can engulf and subsequently provide the necessary niche for the replication of Legionella. Acanthamoebae trophozoites and cysts can harbor and support the replication of unidentified gram-negative bacteria. King has recently shown that bacteria associated with free-living amoebae are more resistant to toxic environments. Assuming that methylotrophic bacteria in situ are a part of a mixed community, and based on our observations that bacteria associated with protozoa may not be easily isolated by standard techniques, we attempted to use protozoa as a tool to isolate TCE-degrading bacteria.
- Research Organization:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Badger Engineers, Inc., Tampa, FL (United States); Solar Energy Research Inst., Golden, CO (United States)
- DOE Contract Number:
- AC05-84OR21400
- OSTI ID:
- 431634
- Report Number(s):
- CONF-900512-; TRN: 96:006510-0078
- Resource Relation:
- Conference: 12. symposium on biotechnology fuels and chemicals, Gatlinburg, TN (United States), 7-11 May 1990; Other Information: PBD: 1991; Related Information: Is Part Of Twelfth symposium on biotechnology for fuels and chemicals; Greenbaum, E. [ed.] [Oak Ridge National Lab., TN (United States)]; Wyman, C.E. [ed.] [Solar Energy Research Inst., Golden, CO (United States)]; PB: 934 p.
- Country of Publication:
- United States
- Language:
- English
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