A comparison of the effects of acetophenone, 1-hexanol, and hexane on S. cerevisiae and Z. mobilis in batch and continuous immobilized-cell culture
Conference
·
OSTI ID:431624
- Univ. of Arkansas, Fayetteville, AR (United States)
The yeast Saccharomyces cerevisiae has traditionally been employed in industrial ethanol production by fermentation because of its rapid specific growth and production rates in the presence of moderately high concentrations of alcohol. More recently, however, the bacterium Zymomonas mobilis has been used with success in laboratory studies. Although product formation by both organisms can be described by the same overall stoichiometric equation, there is an important metabolic difference between the two organisms. S. cerevisiae utilizes the Embden-Meyerhoff-Parnas pathway, which yields 2 mol of ATP/mol of glucose consumed. Z. mobilis, on the other hand, utilizes the Entner-Doudoroff pathway, which yields 1 mol of ATP/mol of glucose consumed. It has been postulated that, at the same specific growth rate, Z. mobilis would consume glucose at a rate at least twice as fast as that of S. cerevisiae, since only half as much energy is produced during the fermentations. The purpose of this paper is to compare the performance of Z mobilis and S cerevisiae in converting glucose to ethanol in the presence of various concentrations of impurities in both batch culture; overall ethanol productivity and column longevity are compared in continuous culture.
- Research Organization:
- Oak Ridge National Lab., TN (United States); Badger Engineers, Inc., Tampa, FL (United States); Solar Energy Research Inst., Golden, CO (United States)
- OSTI ID:
- 431624
- Report Number(s):
- CONF-900512--
- Country of Publication:
- United States
- Language:
- English
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