Effect of immunological inhibition of lysosome function on radiation survival of rat sarcoma cells in culture
An antibody capable of inhibiting lysosomal hydrolytic activity was prepared and its effects on the gamma -radiation survival curves of cultured CCL 47 rat-sarcomal cells were studied over a dose range 0 to 800 rads. In 22 experiments, an average D/sub o/ of 160 rads was determined. The average extrapolation number was 1.7. In 17 experiments in the presence of antilysosomal globulins, an average of D/sub o/ of 200 rads and an extrapolation number of 1.4 were found. It is postulated that these enhanced survival rates were due to the immunologcal suppression of the lysosomal hydrolytic activity. Participation of endogenous acid hydrolases in cell autolysis would be impaired by their insolubilization in situ by the antilysosomal antibodies. A delay in the autolytic events would provide a certain advantage to partially injured cells that retained repair capabilities. (UK)
- Research Organization:
- Boston Univ.
- NSA Number:
- NSA-29-029673
- OSTI ID:
- 4312462
- Journal Information:
- Int. J. Radiat. Biol., v. 25, no. 1, pp. 95-98, Other Information: Orig. Receipt Date: 30-JUN-74; Bib. Info. Source: UK (United Kingdom (sent to DOE from))
- Country of Publication:
- United Kingdom
- Language:
- English
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