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Use of ultraviolet radiation to locate dipicolinic acid in Bacillus cereus spores

Journal Article · · J. Bacteriol., v. 118, no. 1, pp. 202-208
OSTI ID:4312371

A novel method is described that allows a direct determination of the location of a portion of the dipicolinic acid (DPA) in spores. Ultraviolet (uv) irradiation is shown to cause cross-linkage of DPA to spore proteins which have the characteristics of membrane proteins. We suggest that DPA resides in the inner forespore membrane (IFSM) and spore cytoplasm (i.e., the spore protoplast). Only that portion of the DPA in the vicinity of the IFSM appeared to form uv- induced DPA-protein adducts. (auth)

Research Organization:
Commonwealth Scientific and Industrial Research Organization, North Ryde, Australia
NSA Number:
NSA-29-029684
OSTI ID:
4312371
Journal Information:
J. Bacteriol., v. 118, no. 1, pp. 202-208, Journal Name: J. Bacteriol., v. 118, no. 1, pp. 202-208; ISSN JOBAA
Country of Publication:
Country unknown/Code not available
Language:
English