ACTION OF RADIATION ON MAMMALIAN CELLS III. RELATIONSHIP BETWEEN REPRODUCTIVE DEATH AND INDUCTION OF CHROMOSOME ANOMALIES BY X-IRRADIATION OF EUPLOID HUMAN CELLS IN VITRO

Journal Article · Fri Aug 01 04:00:00 EDT 1958 · Proc. Natl. Acad. Sci. U.S.

DOI:https://doi.org/10.1073/pnas.44.8.772· OSTI ID:4309052

Puck, T T

X irradiation of euploid human cells under conditions of stable growth in tissue culture produces chromosomal anomalies whose nature and frequency depend on the dose. Below 50 r the approximate mean lethal dose for cell reproduction in the strains employed practically the only anomalies obtained are those characteristic of single hits. Above this dose range, the incidence of simple breaks is increased, and complex breaks indicative of interaction between two or more hit chromosomes appear and increase with dose. These data support the previously advanced interpretation that the primary process leading to destruction of the reproductive ability of cultured single mammalian cells by x irradiation is a damage to the chromosomes, and they place an upper limit of 40 to 60 r on the value of the dose needed to produce an average of one chromosome abnormality in an euploid, fibroblast-like human cell under these conditions. Pictures of various radiationproduced chromosome breaks, translocations, dicentrics, and ring chromosomes in reproducing, euploid human cells are presented. (auth)

• Research Organization: Univ. of Colorado Medical Center, Denver
• Sponsoring Organization: USDOE
• NSA Number: NSA-12-016120
• OSTI ID: 4309052
• Journal Information: Proc. Natl. Acad. Sci. U.S., Journal Name: Proc. Natl. Acad. Sci. U.S. Vol. Vol: 44
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ANIMAL CELLS
ANIMALS
BIOLOGY AND MEDICINE
CHROMOSOMES
CYTOLOGY
FIBROBLASTS
LETHAL DOSE
MAMMALS
MAN
MITOSIS
MUTATIONS
QUANTITY RATIO
RADIATION DOSES
RADIATION INJURIES
VARIATIONS
X RADIATION