Chlamydia trahomatis in cell culture. I. Comparison of efficiencies of infection in several chemically defined media, at various pH and temperature values, and after exposure to diethylaminoethyl-dextran

Journal Article · Mon Oct 01 04:00:00 EDT 1973 · Appl. Microbiol., v. 26, no. 4, pp. 560-565

OSTI ID:4307538

Rota, T R; Nichols, R L

Three chemically defined cell culture media, Eagle minimum essential medium (MEM) with Earle basal salt solution, Eagle MEM with Hanks basal salt solution, and a modified Eagle MEM, were tested and found capable of supporting the development of Chlamydia trachomatis in /sup 60/Co-treated McCoy cells. The enhancement of trachoma infection by diethylaminoethyl-dextran (DEAE-D) was greater at pH values closer to neutrality than at and other pH values measured at the start of the experiments. Centrifugation of the trachoma inoculum onto cell monolayers at 33 C increased the number of inclusions when compared to centrifugation at 20 C. When the inoculum was centrifuged onto cell monolayers and subsequent incubation was at temperatures ranging from 34 to 39 C, the greatest number of inclusions was observed after incubation from 35 through 37 C. Enhancement of the trachoma infection by DEAE-D was tested at temperatures ranging from 35 to 37 C. These cultures had three- to fivefold increases in inclusions when compared to previously reported experiments in which DEAE-D- treated cultures were incubated at 34 C. (auth)

• Research Organization: Harvard School of Public Health, Boston
• NSA Number: NSA-29-024449
• OSTI ID: 4307538
• Journal Information: Appl. Microbiol., v. 26, no. 4, pp. 560-565, Journal Name: Appl. Microbiol., v. 26, no. 4, pp. 560-565; ISSN APMBA
• Country of Publication: Country unknown/Code not available
• Language: English

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*ANIMAL CELLS-- BIOLOGICAL RADIATION EFFECTS
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GAMMA RADIATION
N48210 --Life Sciences--Radiation Effects on Cells--External Source
N48310* --Life Sciences--Radiation Effects on Microorganisms- -Basic Studies
PHOTON BEAMS
TEMPERATURE DEPENDENCE