X-IRRADIATION OF THE GIANT AMOEBA, PELOMYXA ILLINOISENSIS. II. FURTHER STUDIES ON RECOVERY FOLLOWING SUPRALETHAL EXPOSURE

Journal Article · Tue Apr 01 04:00:00 EST 1958 · J. Exptl. Zool.

DOI:https://doi.org/10.1002/jez.1401370304· OSTI ID:4297025

Daniels, E W

BS>Living, nonirradiated, centrifeged giant amoebae were separated into light and heavy halves which were then injected by fusion into different supralethally x-irradiated cells. Heavy, centrifugal portions of cells centrifuged for 10 minutes nt forces which ranged from 700 g to 30,000 g brought about recovery from supralethal x-ray injury. These heavy portions contained the nuclei and most of the mitochondria. The percentage of light halves capable of preventing death after fusion decreased in a linear manner from the 92% produced by noncentrifuged, nonirradiated whole protoplasm to some 3% produced by light halves centrifuged at 5000 g. Light halves of cells centrifuged at forces grenter than fhis were even less capable of preventing death. The nonirradiated nuclei do not appear to be required by the lethally irradiated cell for recovery providing nonirradiated cytoplasm is present. Exposed nuclei recover and divide repentedly after fnsion. The need of lethally irradiated cells for nonirradiated mitochondria appears to be negligible. Cell division occurred at normal time intervals in the irradiated cells which received nonirradiated heavy hanlves. Many of the light halves centri-fuged at the lower forces also produced this result. The 0.1 to 0.3 mu particulates which do not stain with acidfuchsin, and/or the cytoplasm which contains them, appear to have the capacity to prevent death in supralethally x-irradiated organisms. The migration of these particulates out of the light portions into the heavy portions during centrifugation is closely correlated with the loss in therapeutic value of the light portions. (auth)

• Research Organization: Argonne National Lab., Lemont, Ill.
• Sponsoring Organization: USDOE
• NSA Number: NSA-13-001918
• OSTI ID: 4297025
• Journal Information: J. Exptl. Zool., Journal Name: J. Exptl. Zool. Vol. Vol: 137, No. 3
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

AMOEBA
ANIMAL CELLS
BACTERIA
BIOLOGY AND MEDICINE
CENTRIFUGATION
DYES
MICROORGANISMS
MITOCHONDRIA
MITOSIS
QUANTITY RATIO
RADIATION DOSES
RADIATION INJURIES
RADIATION PROTECTION
RECOVERY
REPRODUCTION
X RADIATION