Hepatic cytochrome P4501A induction in dab (Limanda limanda) after oral dosing with the polychlorinated biphenyl mixture Clophen A40
- Netherlands Inst. for Sea Research, Texel (Netherlands)
- Univ. of Bergen (Norway). Lab. of Marine Molecular Biology
The flatfish dab (Limanda limanda) serves as an indicator species in pollution monitoring programs in the North Sea. The present study investigated the induction response of the monooxygenase system and haematological changes in female dab after multiple administrations of a technical mixture of polychlorinated biphenyls (PCBs). Mature female dab were dosed with 1 mg of the PCB mixture Clophen A40 (Clo A40) in sunflower oil every 6 weeks, with a maximum of three doses per fish. In all PCB-administered groups, levels of cytochrome P4501A (CYPIA) protein, measured with a semi-quantitative ELISA method, and 7-ethoxyresorufin O-deethylase (EROD) activity showed a three- to ninefold induction 14 d after dosing compared with control groups, smaller but also significant increases were observed in total cytochrome P450 ({Sigma} P450) levels. Although the PCB concentrations and the corresponding toxic equivalent (TEQ) value in muscle tissue still increased after administration of the second and third dose of Clo A40, maximum responses of the EROD activity were already reached after the first dose at a TEQ value for chlorinated biphenyls (CB-TEQ) of 2 ng/g lipid. The PCB patterns of liver and muscle tissue of female dab from the central North Sea were found to be virtually identical. Hence, the use of PCB concentrations in muscle as a qualitative model for changes in the liver appears legitimate. Haemoglobin concentrations were elevated after the third dose of Clo A40, whereas haematocrit values and the mean corpuscular haemoglobin concentration (MCHC) between treated and control groups did not differ.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 42929
- Journal Information:
- Environmental Toxicology and Chemistry, Vol. 14, Issue 4; Other Information: PBD: Apr 1995
- Country of Publication:
- United States
- Language:
- English
Similar Records
Biochemical and developmental effects of dietary exposure to polychlorinated biphenyls 126 and 153 in common tern chicks (Sterna hirundo)
Reproductive and MFO effects in crimson-spotted rainbow fish following 3,3{prime},4,4{prime},5-PCB exposure