THE EFFECT OF MASSIVE DOSES OF $gamma$-RADIATION ON THE IMMUNOGENIC PROPERTIES OF BACTERIA OF THE INTESTINAL GROUP
Bacteria of the intestinal group were found to be killed by radiation doses of 400,000 to 600,000 r. When spore forms of bacteria were contained in the material, sterilization was achieved by doses of 1.5 to 2 Mr. Applications of radiosterilization are discussed for the preparation of bacterial-cell vaccines, bacterial antigen complexes. chemical vaccines, and the preparation of vaccines made from bacteria killed by radiation. A study was made of the quality, antigenic and immunogenic properties, liability to retain Vi antigen, and toxicity of vaccines and antigenic complexes prepared from irradiated dysentery and typhoid bacteria. It was found that the radio-antigens were less toxic than antigens prepared from formalinized bacteria or from bacteria which had not been killed before the preparation of the antigen. When antigen previously prepared from formalinized bacteria was subjected to radiation, it either did not differ in toxic properties from the unirradiated antigen or was more toxic. Radiovaccines induced antibody formatdon in the same way as ordinary formalinized vaccines. Experimental data are tabulated. It was concluded that gamma irradiation can be used both for the production of intestinal group vaccines and antigens and for the sterilization of corresponding bacterial preparations already prepared. (C.H.)
- Research Organization:
- Gamaleia Inst. of Epidemiology and Microbiology
- NSA Number:
- NSA-13-007410
- OSTI ID:
- 4280987
- Journal Information:
- J. Microbiol. Epidemiol. Immunobiol. (USSR) (English Translation), Vol. Vol: 29; Other Information: Orig. Receipt Date: 31-DEC-59
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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