LUMINESCENT MICROSCOPY OF THE PERIPHERAL BLOOD IN X IRRADIATION

Journal Article · Sun Mar 01 04:00:00 EST 1959 · Meditsinskaya Radiologiya (U.S.S.R.) For English translation see Med. Radiol., USSR

OSTI ID:4248625

Khodas, M Ya

The possibility of using the method of luminescent microscopy of the peripheral blood for early diagnosis of radiation injury and the evaluation of its severity was investigated. A solution of acridin-orange is used in concentration of 1: 10,000, in 30-minute contact of the blood with fluorochrome. The quantity of leukocytcs, luminescenting a green light, in healthy rats averaged 96. 1%. The effect of single x irradiation (100, 200, 800, and 1,000 r) on the character of luminescence of peripheral blood leukocytes was studied in 4 series of experiments. Irradiation in the above doses produced a qualitativcly identical reaction-a reduction of the quantity of cells, stained in green color, with an increase of the percentage of leukocytes, luminescenting a yellow, orange, and red light. With the increase of the irradiation dose the above effect was more stable and marked. In 13 dogs, subjected to single total irradiation of 600 r there was seen a progressive drop of leukocytes with green luminescence. These data give ground to the assumption that luminescent microscopy of the peripheral blood may serve as one of the indices of the severity of radiation affection. (auth)

• Research Organization: Originating Research Org. not identified
• NSA Number: NSA-13-014200
• OSTI ID: 4248625
• Journal Information: Meditsinskaya Radiologiya (U.S.S.R.) For English translation see Med. Radiol., USSR, Journal Name: Meditsinskaya Radiologiya (U.S.S.R.) For English translation see Med. Radiol., USSR Vol. Vol: 4, No. 3; ISSN MERAA
• Country of Publication: Country unknown/Code not available
• Language: Russian

Related Subjects

BIOLOGY AND MEDICINE
BLOOD
COLOR
DIAGNOSIS
DOGS
DYES
FLUORINE COMPOUNDS
LEUCOCYTES
LUMINESCENCE
MICROSCOPY
QUANTITY RATIO
RADIATION DOSES
RADIATION INJURIES
SOLUTIONS
STABILITY
VARIATIONS
X RADIATION