Natural occurrence of lymphocytes showing cytotoxic activity in BALB/c radiation-induced leukemia RL male 1 cells

Journal Article · Mon Sep 01 04:00:00 EDT 1975 · J. Natl. Cancer Inst., v. 55, no. 3, pp. 603-609

OSTI ID:4182044

Sendo, F; Aoki, T; Boyse, E A; Buafo, C K

Spleen cells from untreated young male and female C57BL/6 and C58 mice and of male C3H/He mice showed cytotoxic activity in vitro against BALB/c x- radiation-induced leukemia RL male 1 cells by $sup 51$Cr-releasing lymphocyte- mediated cytotoxicity (LMC) tests, but old mice of these strains lacked LMC activity. In contrast, spleen cells from male and female AKR, BALB/c, and DBA/2 mice, and from female C3H/He mice had no appreciable LMC activity. The proportion of active cells in spleens from young (C57BL/6 x BALB/c)F$sub 1$ or reciprocal hybrid mice was higher in females than in males. The specificity of the LMC reaction of RL male 1 cells, determined by LMC inhibition assays, was somewhat different from that of previously reported serologic X.1 tests. Thus the antigen detected by LMC has been tentatively designated X.1'. The main effector cells in this system were uncharacterized cells not adherent to glass surfaces or nylon--wool columns. These findings in RL male 1 leukemia extend the evidence for the presence of naturally occurring LMC. With the single unexplained exception of strain C3H/He, the LMC activity against RL male 1 cells, exhibited by untreated mice of various strains, corresponded with a previous classification of mouse strains immunologically as X.1 responders or as X.1 nonresponders according to their ability to reject X.1-positive leukemia cells. (auth)

• Research Organization: National Cancer Inst., Bethesda, MD
• NSA Number: NSA-33-003237
• OSTI ID: 4182044
• Journal Information: J. Natl. Cancer Inst., v. 55, no. 3, pp. 603-609, Journal Name: J. Natl. Cancer Inst., v. 55, no. 3, pp. 603-609; ISSN JNCIA
• Country of Publication: United States
• Language: English

Related Subjects

*LEUKEMIA-- RADIOINDUCTION
*LYMPHOCYTES-- TOXICITY
*SPLEEN CELLS-- TOXICITY
560152* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Animals--Vertebrates
ANTIGENS
MICE
N48520* --Life Sciences--Radiation Effects on Animals-- Vertebrates
SPLEEN
X RADIATION