Inducible error-prone repair in Escherichia coli

Journal Article · Tue Jul 01 00:00:00 EDT 1975 · Proc. Natl. Acad. Sci. U.S.A., v. 72, no. 7, pp. 2753-2757

DOI:https://doi.org/10.1073/pnas.72.7.2753· OSTI ID:4173764

Sedgwick, S G

A hypothesis that ultraviolet-induced mutagenesis arises from the induction of an error-prone mode of postreplication repair that requires the exrA$sup +$ recA$sup +$ genotype has been tested with alkaline sucrose gradient centrifugation coupled with assays of fixation determined by loss of photoreversibility. The inhibitor of protein synthesis, chloramphenicol, added before irradiation, prevented a small amount of postreplication repair and completely eliminated mutation fixation in E. coli WP2/sub s/ uvrA. However, chloramphenicol did not affect strand joining: in uvrA bacteria allowed 20 min of growth between irradiation and antibiotic treatment; in nonmutable uvrA exrA bacteria; and in urvA tif bacteria grown at 42$sup 0$ for 70 min before irradiation. These observations indicate that an inducible product is involved in a fraction of postreplication repair and is responsible for induced mutagenesis. (auth)

• Research Organization: Brookhaven National Lab., Upton, NY
• Sponsoring Organization: USDOE
• NSA Number: NSA-33-000878
• OSTI ID: 4173764
• Journal Information: Proc. Natl. Acad. Sci. U.S.A., v. 72, no. 7, pp. 2753-2757, Journal Name: Proc. Natl. Acad. Sci. U.S.A., v. 72, no. 7, pp. 2753-2757; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

*ANTIBIOTICS-- BIOLOGICAL EFFECTS
*BIOLOGICAL REPAIR-- BIOLOGICAL RADIATION EFFECTS
*CHLORAMPHENICOL-- BIOLOGICAL EFFECTS
*ESCHERICHIA COLI-- MUTAGENESIS
560131* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Microorganisms--Basic Studies
N48310* --Life Sciences--Radiation Effects on Microorganisms- -Basic Studies
ULTRAVIOLET RADIATION