Fluorescence and bioluminescence of bacterial luciferase intermediates

Journal Article · Tue Oct 21 04:00:00 EDT 1975 · Biochemistry, v. 14, no. 21, pp. 4719-4723

DOI:https://doi.org/10.1021/bi00692a024· OSTI ID:4164537

Balny, C; Hastings, J W

An intermediate in the luciferase-catalyzed bioluminescent oxidation of FMNH$sub 2$, isolated and purified by chromatography at --20$sup 0$, was postulated to be an oxygenated reduced flavine-luciferase. Maintained and studied at --20 to --30$sup 0$, this material exhibits a relatively weak fluorescence emission peaking at about 505 nm when excited at 370 nm. It may comprise more than one species. Upon continued exposure to light at 370 nm, the intensity of this fluorescence increases, often by a factor of 5 or more, and its emission spectrum is blue shifted to a maximum at about 485 nm. Upon warming this fluorescence is lost and the fluorescence of flavine mononucleotide appears. If warming is carried out in the presence of a long chain aldehyde, bioluminescence occurs, with the appearance of a similar amount of flavine fluorescence. The bioluminescence yield is about the same with irradiated and nonirradiated samples. The bioluminescence emission spectrum corresponds exactly to the fluorescence emission spectrum of the intermediate formed by irradiation, implicating the latter as being structurally close to the emitting species in bioluminescence. (auth)

• Research Organization: Ecole des Hautes Etudes, Paris
• Sponsoring Organization: USDOE
• NSA Number: NSA-33-003161
• OSTI ID: 4164537
• Journal Information: Biochemistry, v. 14, no. 21, pp. 4719-4723, Journal Name: Biochemistry, v. 14, no. 21, pp. 4719-4723; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

*BACTERIA-- METABOLISM
*FLUORESCENCE-- BIOLOGICAL RADIATION EFFECTS
*LUCIFERASE-- LUMINESCENCE
*LUMINESCENCE-- BIOLOGICAL RADIATION EFFECTS
560111* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Biochemicals--In Vitro
CATALYSIS
FLAVINES
N48110* --Life Sciences--Radiation Effects on Biochemicals-- In-Vitro
NUCLEOTIDES
OXIDATION
SPECTRA
TEMPERATURE DEPENDENCE
ULTRAVIOLET RADIATION