TRITIUM-LABELED COMPOUNDS. III. ALDOSES-1-t

Journal Article · Tue Mar 01 04:00:00 EST 1960 · J. Research Natl. Bur. Standards

DOI:https://doi.org/10.6028/jres.064A.018· OSTI ID:4162113

Isbell, H S; Frush, H L; Holt, N B; Moyer, J D

>In a new method for preparing aldoses labeled with tritium at carbon 1, the aldonic lactone is reduced with lithium borohydride-t in pyridine solution. The method is suitable for preparing aldoses-1-t having high specific activities. The crude product contains a substantial proportion of the corresponding alditol- 1-t, but the pure aldose-1-t is readily separated by fractional recrystallization or paper chromatography. By means of an isotope-dllution technique, yields were determined for the following aldoses-1-t and the corresponding alditols-1-t: D- arabinose-1-t, D-xylose-1-t, D-ribose-1-t, D-glucose-1-t, D-galactose-1-t, D- mannose-1-t, L-rhamnose-1-t, maltose1-t, and lactose-1-t. These labeled materials were prepared by reducing the corresponding lactones with sodium amalgam in tritiated water. Although this latter method is not suitable for preparing labeled aldoses of high specific acttvity, the products are more readily purified than those obtained by reducing the lactones with lithium borohydride-t. D-Glucose-1-t, obtained by each of these reduction methods, was oxidized with bromine, and the resulting D-gluconic acid was found to be nonradioactive. In the samples oxidized, tritium was present only at Cl. An apparatus used for reclaiming tritiated water by freeze-drying is depicted; it incorporates an efficient device for trapping entrained solids or liquids. (auth)

• Research Organization: Originating Research Org. not identified
• Sponsoring Organization: USDOE
• NSA Number: NSA-14-013677
• OSTI ID: 4162113
• Journal Information: J. Research Natl. Bur. Standards, Journal Name: J. Research Natl. Bur. Standards Vol. Vol: 64A
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ARABINOSE
BOROHYDRIDES
BROMINE
CARBOHYDRATES
CHEMISTRY
CHROMATOGRAPHY
ESTERS
GLUCOSE-D
IMPURITIES
INSTRUMENTS
ISOMERS
ISOTOPE DILUTION
ISOTOPES
LABELLED COMPOUNDS
LACTONES
LACTOSE
LITHIUM COMPOUNDS
LITHIUM HYDRIDES
MALTOSE
MANNOSE
MERCURY ALLOYS
ORGANIC ACIDS
OXIDATION
PLANNING
PREPARATION
PYRIDINES
REDUCTION
SODIUM ALLOYS
SOLUTIONS
SUGARS
TRAPS
TRITIUM
XYLOSE