EFFECTS OF IN VITRO RADIOCOBALT IRRADIATION OF RABBIT OVA ON SUBSEQUENT DEVELOPMENT IN VIVO WITH SPECIAL REFERENCE TO THE IRRADIATION OF MATERNAL ORGANISM
Fertilized rabbit ova recovered one to six days after mating were irradiated in vitro from a radiocobalt source and then transplanted into recipient animals. When examined 22 to 28 days later 44, 33, 8 and 0% of ova irradiated respectively at 50, 100, 1,000 and 5,000 r developed into apparently normal fetuses without external or internal malformation. No significant differential sensitivity was apparent in ova irradiated at different ages. It was found further that 34, 36, 19 and 10% of two-, 4-, and 6-day ova irradiated respectively in vitro at 200, 400, 600, and 800 r developed into "normal" fetuses. Again no malformation of fetuses and no differential radiosensitivity between ova of different ages were observed. Following whole body irradiation at 400 r, it was found that 40% of non-irradiated ova developed into normal fetuses when transplanted into recipient animals that had been irradiated (vs. 36% in the irradiation of ova alone). However, only 17% of estimated ova developed into "normal" fetuses when pregnant rabbits were irradiated 2, 4 or 6 days after insemination (vs. 64% in the control). It appears that irradiation of the maternal organism influences embryonic development and that irradiation of pregnant animals exerts a combination of ill effects, on the ova and on their environment. Cytological study of irradiated blastocysts revealed no chromosomal breakage immediately after irradiation. Chromosomal abnormalities, fragmentation and condensation of chromatin were observed during the culture of irradiated blastocysts in accordance with the dosages applied. From this study it is concluded that (1) although 50 r may affect embryonic development, there seems to be no differential effect up to 400 r, above which greater prenatal death occurs; (2) before implantation, irradiated ova either die or develop into apparently normal fetuses and there is no evidence of differential radiosensitivity at various stages of development; (3) irradiation of the maternal organism alone also affects embryonic development; and (4) radiation damage affects a fundamental biological system which leads to the nuclear damage and failure of mitosis, and the death of ova. (auth)
- Research Organization:
- Worcester Foundation for Experimental Biology, Shrewsbury, Mass.
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-14-023945
- OSTI ID:
- 4158391
- Journal Information:
- Anat. Record, Vol. Vol: 137; Other Information: Orig. Receipt Date: 31-DEC-60
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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Related Subjects
AGE
BIOLOGY
BODY
CELL NUCLEI
CHROMOSOMES
COBALT ISOTOPES
CYTOLOGY
EMBRYOS
FETUSES
GONADS
IRRADIATION
LETHAL DOSE
MALFORMATIONS
MEIOSIS
MITOSIS
NUCLEIC ACIDS
PREGNANCY
RABBITS
RADIATION DOSES
RADIATION EFFECTS
RADIATION INJURIES
RADIATION SOURCES
RADIOSENSITIVITY
REPRODUCTION
TRANSPLANTS
VARIATIONS